Evaluation of hypoxia-inducible factor-1α and urine non-transferrin-bound iron concentrations in cats with chronic kidney disease.

ABSTRACT:

Introduction

Hypoxia-inducible factors (HIF) regulate gene transcription, which aids hypoxia adaptation while promoting renal fibrosis. Non-transferrin-bound iron (NTBI) is a catalytic form of iron that can lead to oxidative damage. However, NTBI in cat biofluids has rarely been evaluated.

Aims

We assessed cat plasma and urine HIF-1α (pHIF-1α/uHIF-1α) concentrations and urine NTBI (uNTBI) concentrations to investigate their relationship with chronic kidney disease (CKD) severity.

Methods

pHIF-1α and uHIF-1α concentrations were measured using commercial ELISA kits, while uNTBI concentrations were detected by inductively coupled plasma mass spectrometry.

Results

Healthy cats (n = 35) and cats with CKD (n = 84) formed the study cohorts. pHIF-1α conce

SUBMITTER: Chen CH 

PROVIDER: S-EPMC11694447 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature