Genome-wide detection and analysis of cell wall-bound proteins with LPxTG-like sorting motifs.

ABSTRACT: Surface proteins of gram-positive bacteria often play a role in adherence of the bacteria to host tissue and are frequently required for virulence. A specific subgroup of extracellular proteins contains the cell wall-sorting motif LPxTG, which is the target for cleavage and covalent coupling to the peptidoglycan by enzymes called sortases. A comprehensive set of putative sortase substrates was identified by in silico analysis of 199 completely sequenced prokaryote genomes. A combination of detection methods was used, including secondary structure prediction, pattern recognition, sequence homology, and genome context information. With the hframe algorithm, putative substrates were identified that could not be detected by other methods due to errors in open reading frame calling, frameshifts

SUBMITTER: Boekhorst J 

PROVIDER: S-EPMC1169495 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature