APEX-based proximity labeling in Plasmodium identifies a membrane protein with dual functions during mosquito infection.

ABSTRACT: Transmission of the malaria parasite Plasmodium to mosquitoes necessitates gamete egress from red blood cells to allow zygote formation and ookinete motility to enable penetration of the midgut epithelium. Both processes are dependent on the secretion of proteins from distinct sets of specialized vesicles. Inhibiting some of these proteins has shown potential for blocking parasite transmission to the mosquito. To identify new transmission blocking vaccine candidates, we aimed to define the microneme content from ookinetes of the rodent model organism Plasmodium berghei using APEX2-mediated rapid proximity-dependent biotinylation. Besides known proteins of ookinete micronemes, this identified over 50 novel candidates and sharpened the list of a previous survey based on subcellular fractiona

SUBMITTER: Kehrer J 

PROVIDER: S-EPMC11695019 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature