Quantitative sampling by IR-MALDESI is not susceptible to tissue heterogeneity in a multi-organ model.

ABSTRACT: Quantitative mass spectrometry imaging (qMSI) provides the relative or absolute analyte quantities in a biological specimen in a spatially resolved manner. However, the chemical complexity and physical structure of biological specimens often require one to precisely account for matrix effects in qMSI platforms. Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) completely ablates a volume of cryosectioned tissue. This enables the use of a normalization standard that is sprayed underneath the tissue for qMSI applications. Complete sampling has shown to be a significant advantage for qMSI by IR-MALDESI; however, the impact of high tissue heterogeneity has not been systematically studied or quantified. The bias introduced by tissue heterogeneity was investigated by

SUBMITTER: Joignant AN 

PROVIDER: S-EPMC11695138 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature