A new-to-nature photosynthesis system enhances utilization of one-carbon substrates in Escherichia coli.

ABSTRACT: Photosynthesis harvests solar energy to convert CO₂ into chemicals, offering a potential solution to reduce atmospheric CO₂. However, integrating photosynthesis into non-photosynthetic microbes to utilize one-carbon substrates is challenging. Here, a photosynthesis system is reconstructed in E. coli, by integrating light and dark reaction to synthesize bioproducts from one-carbon substrates. A light reaction is reconstructed using the photosystem of photosynthetic bacteria, increasing ATP and NADH contents by 337.9% and 383.7%, respectively. A dark reaction is constructed by designing CO₂ fixation pathway to synthesize pyruvate. By assembling the light and dark reaction, a photosynthesis system is established and further programmed by installing an energy a

SUBMITTER: Tong T 

PROVIDER: S-EPMC11695776 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature