Single-cell RNA sequencing identifies CXADR as a fate determinant of the placental exchange surface.

ABSTRACT: The placenta is the critical interface between mother and fetus, and consequently, placental dysfunction underlies many pregnancy complications. Placental formation requires an adequate expansion of trophoblast stem and progenitor cells followed by finely tuned lineage specification events. Here, using single-cell RNA sequencing of mouse trophoblast stem cells during the earliest phases of differentiation, we identify gatekeepers of the stem cell state, notably Nicol1, and uncover unsuspected trajectories of cell lineage diversification as well as regulators of lineage entry points. We show that junctional zone precursors and precursors of one of the two syncytial layers of the mouse placental labyrinth, the Syncytiotrophoblast-I lineage, initially share similar trajectories. Importantly,

SUBMITTER: Angelova DM 

PROVIDER: S-EPMC11695997 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature