Rapid detection of the invasive tomato leaf miner, Phthorimaea absoluta using simple template LAMP assay.

ABSTRACT: The tomato leaf miner (TLM), Phthorimaea absoluta Meyrick, 1917 (Lepidoptera: Gelechiidae) is a destructive invasive insect that has expanded its global distribution. Rapid and accurate identification of invasive pests is essential to support subsequent management and devise control measures. To accurately diagnose P. absoluta, a Loop Mediated Isothermal Amplification (LAMP) assay (TLM-LAMP) was developed to amplify the target region of mitochondrial cytochrome oxidase subunit I (COI) gene. The TLM-LAMP assay can identify the P. absoluta within 60 min at 65 °C after sample extraction. Cross-reactivity analysis against three closely related non-target species, Phthorimaea operculella (Zeller, 1873), Pectinophora gossypiella (Saunders, 1844), and Aproaerema modicella (Deventer, 1904) confirm

SUBMITTER: Kumar A 

PROVIDER: S-EPMC11696019 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature