Attomolar-sensitive milk fever sensor using 3D-printed multiplex sensing structures.

ABSTRACT: The diagnosis of milk fever or hypocalcemia in lactating cows has a significant economic impact on the dairy industry. It is challenging to identify asymptomatic subclinical hypocalcemia (SCH) in transition dairy cows. Monitoring subclinical hypocalcemia in milk samples can expedite treatment and improve the health, productivity, and welfare of dairy cows. In this study, an attomolar-sensitive sensor is developed using extrusion-based 3D-printed sensing structures to detect the ratio of ionized calcium to phosphate levels in milk samples. The unique geometries of the lateral structure of 3D-printed sensors, along with the wrinkled surfaces, provide a limit of detection down to the attomole (138 aM) concentration of the target analyte. The calcium-to-phosphate ratio in milk samples not only

SUBMITTER: Ataei Kachouei M 

PROVIDER: S-EPMC11696081 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature