PathCrisp: an innovative molecular diagnostic tool for early detection of NDM-resistant infections.

ABSTRACT: The rapid and early detection of infections and antibiotic resistance markers is a critical challenge in healthcare. Currently, most commercial diagnostic tools for analyzing antimicrobial resistance patterns of pathogens require elaborate culture-based testing. Our study aims to develop a rapid, accurate molecular detection system that can be used directly from culture, thereby introducing molecular testing in conjunction with culture tests to reduce turnaround time and guide therapy. PathCrisp assay, a combination of loop-mediated isothermal amplification and CRISPR-based detection, maintained at a single temperature, was designed and tested on clinical isolates. The specificity and sensitivity of the assay was analyzed, post which the assay was compared with the polymerase chain reactio

SUBMITTER: Patil S 

PROVIDER: S-EPMC11696219 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature