Scalable fabrication of an array-type fixed-target device for automated room temperature X-ray protein crystallography.

ABSTRACT: X-ray crystallography is one of the leading tools to analyze the 3-D structure, and therefore, function of proteins and other biological macromolecules. Traditional methods of mounting individual crystals for X-ray diffraction analysis can be tedious and result in damage to fragile protein crystals. Furthermore, the advent of multi-crystal and serial crystallography methods explicitly require the mounting of larger numbers of crystals. To address this need, we have developed a device that facilitates the straightforward mounting of protein crystals for diffraction analysis, and that can be easily manufactured at scale. Inspired by grid-style devices that have been reported in the literature, we have developed an X-ray compatible microfluidic device that can be used to trap protein crystals

SUBMITTER: Saha S 

PROVIDER: S-EPMC11696357 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature