Digital PCR detection of microsporidia in household pipe water of patients with microsporidial keratitis.

ABSTRACT: This is a prospective non-comparative experimental study aimed to investigate the presence of microsporidia in household pipe water of microsporidial keratitis (MK) patients and to describe the patients' characteristics and behavioral risk factors. This study was conducted at Walailak University Hospital, Thailand, from July 2022 to February 2024. Patients diagnosed with MK were surveyed using a questionnaire to assess personal behaviors and source of household water. Water from faucets of MK patients' houses were collected for laboratory analysis with digital polymerase chain reaction (dPCR) to detect microsporidia in water samples. The study involved 54 MK patients, with 23 households contributing water samples. Utilizing dPCR analysis, we found that all water samples were contaminated w

SUBMITTER: Sriyuttagrai W 

PROVIDER: S-EPMC11696575 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature