Arctiin alleviates the progression of osteoarthritis by regulating the cholesterol metabolic pathway.

ABSTRACT: Osteoarthritis (OA) is a multi-factorial degenerative joint disease with unclear pathogenesis. Conservative treatments, primarily aimed at pain relief, fail to halt disease progression. Metabolic syndrome has recently been implicated in OA pathogenesis, underscoring the need for novel therapeutic strategies. Arctiin (ARC), a lignan known for its anti-inflammatory and anti-osteoporotic properties, has potential effects on OA that merit exploration. We assessed ARC's impact on chondrocyte viability using the Cell Counting Kit-8 and toluidine blue staining for glycosaminoglycan presence. Gene and protein expression were analyzed via RT-PCR, Western blotting, and immunofluorescence. An OA rat model was employed for in vivo evaluations through histological assessments and micro-CT scanning. ARC

SUBMITTER: Mai J 

PROVIDER: S-EPMC11696608 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature