Protein stabilization in spray drying and solid-state storage by using a 'molecular lock' - exploiting bacterial adaptations for industrial applications.

ABSTRACT: Small, stable biomedicines, like peptides and hormones, are already available on the market as spray dried formulations, however large biomolecules like antibodies and therapeutic enzymes continue to pose stability issues during the process. Stresses during solid-state formation are a barrier to formulation of large biotherapeutics as dry powders. Here, we explore an alternative avenue to protein stabilisation during the spray drying process, moving away from the use of excipients. In thermophilic proteins, the presence of C-termini extensions can add to their stability by increasing molecular rigidity. Hence, we explored a unique thermostable amino acid extension in the C-terminal of an aldehyde dehydrogenase tetramer originating from Thermus thermophilus HB27 (ALDHTt), and

SUBMITTER: Brytan W 

PROVIDER: S-EPMC11696676 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature