Enhancers and genome conformation provide complex transcriptional control of a herpesviral gene.

ABSTRACT: Complex transcriptional control is a conserved feature of both eukaryotes and the viruses that infect them. Despite viral genomes being smaller and more gene dense than their hosts, we generally lack a sense of scope for the features governing the transcriptional output of individual viral genes. Even having a seemingly simple expression pattern does not imply that a gene's underlying regulation is straightforward. Here, we illustrate this by combining high-density functional genomics, expression profiling, and viral-specific chromosome conformation capture to define with unprecedented detail the transcriptional regulation of a single gene from Kaposi's sarcoma-associated herpesvirus (KSHV). We used as our model KSHV ORF68 - which has simple, early expression kinetics and is essential for

SUBMITTER: Morgens DW 

PROVIDER: S-EPMC11696879 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature