H and B Blood Antigens Are Essential for In Vitro Replication of GII.2 Human Norovirus.

ABSTRACT:

Background

Human norovirus (HuNoV) is a major cause of enteric infectious gastroenteritis and is classified into several genotypes based on its capsid protein amino acid sequence and nucleotide sequence of the polymerase gene. Among these, GII.4 is the major genotype worldwide. Epidemiological studies have highlighted the prevalence of GII.2. Although recent advances using human tissue- and induced pluripotent stem cell (iPSC)-derived intestinal epithelial cells (IECs) have enabled in vitro replication of multiple HuNoV genotypes, GII.2 HuNoV could replicate only in tissue-derived IECs and not in iPSC-derived IECs.

Methods

We investigated the factors influencing GII.2 HuNoV replication in IECs, focusing on histo-blood group antigens. We also assessed the immunogenicity of GI

SUBMITTER: Tamiya S 

PROVIDER: S-EPMC11697099 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature