Cep78 knockout causes sterility and oligoasthenoteratozoospermia in male mice.

ABSTRACT: Oligoasthenoteratozoospermia (OAT) is a common cause of infertility among males, and the majority of cases of idiopathic OAT are thought to be attributed to genetic defects. In this study, the role of the CEP78 protein in spermatogenesis was initially investigated using Cep78 knockout (Cep78^-/-) mice. Notably, the male Cep78^-/- mice exhibited the OAT phenotype and sterility. To elucidate the mechanisms underlying the functions of the Cep78 gene in spermatogenesis, the histomorphology of germ cells was investigated during different stages of mitosis, meiosis, and spermiogenesis. Apoptotic assays and RNA-sequencing analyses were additionally performed using the testicular tissue samples of control and Cep78^-/- mice. The findings strongly suggested that defect

SUBMITTER: Liu M 

PROVIDER: S-EPMC11697357 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature