Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel.

ABSTRACT: The innate and adaptive immune systems, though often studied separately, interact deeply and respond to stimuli simultaneously, with leukocytes displaying a range of pro- to anti-inflammatory phenotypes. This protocol details a procedure for characterizing murine innate and adaptive immune phenotypes using a 40-color full-spectral flow cytometry panel. We describe steps for organ collection, sample preparation, immunofluorescent staining, and acquisition to reproducibly and cost-effectively study tissue-resident leukocytes, their subpopulations, and inflammatory status in various organs.

SUBMITTER: Lemaitre P 

PROVIDER: S-EPMC11697560 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature