Non-coding RNAs secreted by renal cancer include piR_004153 that promotes migration of mesenchymal stromal cells.

ABSTRACT:

Background

Renal cell cancer (RCC) is the most common and highly malignant subtype of kidney cancer. Mesenchymal stromal cells (MSCs) are components of tumor microenvironment (TME) that influence RCC progression. The impact of RCC-secreted small non-coding RNAs (sncRNAs) on TME is largely underexplored. Here, we comprehensively analysed the composition of exosomal sncRNAs secreted by RCC cells to identify those that influence MSCs.

Methods

Exosomal sncRNAs secreted by RCC cells and normal kidney cells were analyzed using RNAseq, followed by qPCR validation. MSCs were treated by conditioned media (CM) derived from RCC cells and transfected with piRNA, followed by the analysis of proliferation, viability, migration and immunocytochemical detection of piRNA. Expression of MSCs

SUBMITTER: Boguslawska J 

PROVIDER: S-EPMC11697636 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature