Production of site-specific antibody conjugates using metabolic glycoengineering and novel Fc glycovariants.

ABSTRACT: Molecular conjugation to antibodies has emerged as a growing strategy to combine the mechanistic activities of the attached molecule with the specificity of antibodies. A variety of technologies have been applied for molecular conjugation; however, these approaches face several limitations, including disruption of antibody structure, destabilization of the antibody, and/or heterogeneous conjugation patterns. Collectively, these challenges lead to reduced yield, purity, and function of conjugated antibodies. While glycoengineering strategies have largely been applied to study protein glycosylation and manipulate cellular metabolism, these approaches also harbor great potential to enhance the production and performance of protein therapeutics. Here, we devise a novel glycoengineering workflo

SUBMITTER: Bernstein ZJ 

PROVIDER: S-EPMC11697773 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature