Proteomic characterization of molecular mechanisms of paraquat-induced lung injury in a mouse model.

ABSTRACT:

Background

We sought to explore the molecular mechanisms underpinning acute lung injury (ALI) caused by poisoning with paraquat (PQ).

Methods

Selection mice were intraperitoneally injected with PQ at 40 mg/kg, whereas controls were injected with sterile saline. On days 2, 7, and 14 after administration, mice were anesthetized and sacrificed, and lung tissue was removed. Lung pathological changes were observed with conventional staining techniques. Lung tissue components were assessed with tandem mass spectrometry tag technology, and differentially expressed proteins (DEPs) were bioinformatically analyzed and investigated with parallel reaction monitoring.

Results

The expression of 91, 160, and 78 proteins was significantly altered at days 2, 7, and 14, respectively. G

SUBMITTER: Zhou YQ 

PROVIDER: S-EPMC11697879 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature