CYP3A5 promotes glioblastoma stemness and chemoresistance through fine-tuning NAD⁺/NADH ratio.

ABSTRACT:

Background

Glioblastoma multiforme (GBM) exhibits a cellular hierarchy with a subpopulation of stem-like cells known as glioblastoma stem cells (GSCs) that drive tumor growth and contribute to treatment resistance. NAD(H) emerges as a crucial factor influencing GSC maintenance through its involvement in diverse biological processes, including mitochondrial fitness and DNA damage repair. However, how GSCs leverage metabolic adaptation to obtain survival advantage remains elusive.

Methods

A multi-step process of machine learning algorithms was implemented to construct the glioma stemness-related score (GScore). Further in silico and patient tissue analyses validated the predictive ability of the GScore and identified a potential target, CYP3A5. Loss-of-function or gain-of-func

SUBMITTER: Hu W 

PROVIDER: S-EPMC11697892 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature