Immunoregulatory Macrophages Modify Local Pulmonary Immunity and Ameliorate Hypoxic Pulmonary Hypertension.

ABSTRACT:

Background

Macrophages play a significant role in the onset and progression of vascular disease in pulmonary hypertension, and cell-based immunotherapies aimed at treating vascular remodeling are lacking. We aimed to evaluate the effect of pulmonary administration of macrophages modified to have an anti-inflammatory/proresolving phenotype in attenuating early pulmonary inflammation and progression of experimentally induced pulmonary hypertension.

Methods

Mouse bone marrow-derived macrophages were polarized in vitro to a regulatory (M2_reg) phenotype. M2_reg profile and anti-inflammatory capacity were assessed in vitro upon lipopolysaccharide/IFNγ (interferon-γ) restimulation, before their administration to 8- to 12-week-old mice. M2_reg protecti

SUBMITTER: Fernandez-Gonzalez A 

PROVIDER: S-EPMC11697987 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature