Transcriptomic DN3 clock neuron subtypes regulate <i>Drosophila</i> sleep.

ABSTRACT: Circadian neurons within animal brains orchestrate myriad physiological processes and behaviors, but the contribution of these neurons to the regulation of sleep is not well understood. To address this deficiency, we leveraged single-cell RNA sequencing to generate a comprehensive census of transcriptomic cell types of Drosophila clock neurons. We focused principally on the enigmatic DN3s, which constitute most fly brain clock neurons and were previously almost completely uncharacterized. These DN3s are organized into 12 clusters with unusual gene expression features compared to the more well-studied clock neurons. We further show that previously uncharacterized DN3 subtypes promote sleep through a G protein-coupled receptor, TrissinR. Our findings indicate an intricate regul

SUBMITTER: Ma D 

PROVIDER: S-EPMC11698076 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature