Ribosomes translocation into the spore of Bacillus subtilis is highly organised and requires peptidoglycan rearrangements.

ABSTRACT: In the spore-forming bacterium Bacillus subtilis transcription and translation are uncoupled and the translational machinery is located at the cell poles. During sporulation, the cell undergoes morphological changes including asymmetric division and chromosome translocation into the forespore. However, the fate of translational machinery during sporulation has not been described. Here, using microscopy and mass spectrometry, we show the localisation of ribosomes during sporulation in wild type and mutant Bacillus subtilis. We demonstrate that ribosomes are associated with the asymmetric septum, a functionally important organelle in the cell's developmental control, and that SpoIIDMP-driven peptidoglycan rearrangement is crucial for ribosomes packing into the forespore. We also show that th

SUBMITTER: Iwanska O 

PROVIDER: S-EPMC11698733 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature