Unsupervised clustering reveals noncanonical myeloid cell subsets in the brain tumor microenvironment.

ABSTRACT: The tumor immune microenvironment (TiME) of human central nervous system (CNS) tumors remains to be comprehensively deciphered. Here, we employed flow cytometry and RNA sequencing analysis for a deep data-driven dissection of a diverse TiME and to uncover noncanonical immune cell types in human CNS tumors by using seven tumors from five patients. Myeloid subsets comprised classical microglia, monocyte-derived macrophages, neutrophils, and two noncanonical myeloid subsets: CD3⁺ myeloids and CD19⁺ myeloids. T lymphocyte subsets included double-negative (CD4^- CD8^-) T cells (DNTs). Noncanonical myeloids and DNTs were explored on independent datasets, suggesting that our DNT phenotype represents γδ T cells. Noncanonical myeloids were validated using o

SUBMITTER: Hermelo I 

PROVIDER: S-EPMC11699035 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature