Characterization and evaluation of the immobilized laccase enzyme potential in dye degradation via one factor and response surface methodology approaches.

ABSTRACT: In the current study, calcium alginate was used as a carrier for Agaricus bisporus CU13 laccase immobilization, with an immobilization yield of the entrapped laccase of 91.95%. Free and immobilized enzymes showed their best enzyme activity at 60 °C as an optimum temperature. Free laccase was nearly completely inactivated at high temperatures (70 and 80 °C C) after 30 min, whereas the immobilized form retained around 60 and 40% of its activity after 30 min at the same temperatures. The metal ion of MgSO₄ showed the best impact on the Agaricus bisporus laccase activity with a relative activity of 113.1 and 106.8% at concentrations of 10 mM and 2.5 mM, respectively. The best Cibacron D-Blue SGL dye degradation by laccase enzyme was obtained at pH 6.0, 0.354 U of laccase, and 100 mg

SUBMITTER: Othman AM 

PROVIDER: S-EPMC11699123 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature