Evaluating Nanotrap Microbiome Particles as A Wastewater Viral Concentration Method.

ABSTRACT: Wastewater-based surveillance has emerged as a powerful approach to monitoring infectious diseases within a community. Typically, wastewater samples are concentrated before viral analyses to improve sensitivity. Current concentration methods vary in time requirements, costs, and efficiency. Here, we evaluated the concentration efficiency and bias of a novel viral concentration approach, Nanotrap Microbiome Particles (NMP), in wastewater. NMP concentration efficiency was target-specific, with significantly lower concentrations of the bacterial indicator HF183 and viral indicator Carjivirus (formerly crAssphage) relative to direct extraction (1.2 × 10⁵ vs. 3.4 × 10⁵ GC/mL and 2.0 × 10⁵ vs. 1.2 × 10⁵ GC/mL, respectively), but significantly higher co

SUBMITTER: Shaffer M 

PROVIDER: S-EPMC11700038 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature