Bioinformatics in vivo and in vitro assays identified miR-486-5p as a tumor suppressor miRNA in hepatocellular carcinoma.

ABSTRACT:

Background

This study aimed to explore key microRNAs (miRNAs) and their effects on hepatocellular carcinoma (HCC) progression.

Methods

Key deregulated miRNAs in HCC were screened from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. The anti-cancer effects of miR-486-5p were validated using a cell counting kit-8 assay, flow cytometry, scratch assay, transwell assay, and an orthotopic transplantation tumor model. Furthermore, the expression, clinical significance, and function of miR-486-5p and its targets were predicted using bioinformatics. Additionally, a luciferase reporter assay was performed to validate the miR-486-5p target.

Results

By integrating multiple datasets from TCGA and GEO databases, we identified miR-486-5p as the only lowly

SUBMITTER: Li X 

PROVIDER: S-EPMC11700245 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature