Kinetic network modeling with molecular simulation inputs: A proton-coupled phosphate symporter.

ABSTRACT: Phosphate, an essential metabolite involved in numerous cellular functions, is taken up by proton-coupled phosphate transporters of plants and fungi within the major facilitator family. Similar phosphate transporters have been identified across a diverse range of biological entities, including various protozoan parasites linked to human diseases, breast cancer cells with increased phosphate requirements, and osteoclast-like cells engaged in bone resorption. Prior studies have proposed an overview of the functional cycle of a proton-driven phosphate transporter (PiPT), yet a comprehensive understanding of the proposed reaction pathways necessitates a closer examination of each elementary reaction step within an overall kinetic framework. In this work, we leverage kinetic network modeling in

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11700355 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature