Zinc inhibits the voltage-gated proton channel HCNL1.

ABSTRACT: Voltage-gated ion channels allow ion flux across biological membranes in response to changes in the membrane potential. HCNL1 is a recently discovered voltage-gated ion channel that selectively conducts protons through its voltage-sensing domain (VSD), reminiscent of the well-studied depolarization-activated Hv1 proton channel. However, HCNL1 is activated by hyperpolarization, allowing the influx of protons, which leads to an intracellular acidification in zebrafish sperm. Zinc ions (Zn²⁺) are important cofactors in many proteins and essential for sperm physiology. Proton channels such as Hv1 and Otopetrin1 are inhibited by Zn²⁺. We investigated the effect of Zn²⁺ on heterologously expressed HCNL1 channels using electrophysiological and fluorometric techniq

SUBMITTER: Kuwabara MF 

PROVIDER: S-EPMC11700363 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature