Mice carrying the full-length human immunoglobulin loci produce antigen-specific human antibodies with the lambda light chain.

ABSTRACT: The development of antibody drugs through animal immunization typically requires the humanization of host antibodies to address concerns about immunogenicity in humans. However, employing an animal model capable of producing human antibodies presents the opportunity to develop antibody drugs without the need for humanization. Despite the ratio of human immunoglobulin (Ig) κ to Igλ usage being approximately 60%:40%, the majority of approved antibody therapeutics are kappa antibodies, and the development of lambda antibodies as therapeutic agents has lagged behind. Therefore, in this study, we developed mice carrying the IGH and IGL loci (IGHL), which can produce human lambda antibodies, using mouse artificial chromosome (MAC) vectors. We demonstrated that IGHL mice consistentl

SUBMITTER: Shimoya K 

PROVIDER: S-EPMC11700626 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature