Sample-to-answer detection of miRNA from whole blood using thermally responsive alkane partitions.

ABSTRACT: Circulating miRNA offers a tremendous opportunity as a biomarker paradigm for many applications in disease diagnostics, including point-of-care diagnostics for global health needs. However, despite the numerous miRNA detection schemes reported, there still does not exist a solution for highly sensitive sample-to-answer detection of miRNA directly from complex samples, such as whole blood. We recently developed thermally responsive alkane partitions (TRAPs), which - when combined with magnetic microbeads - enable the complete assay automation from whole blood. Here we apply TRAPs with ligation-LAMP to automate the detection of miRNA in whole blood samples. MBs and a TRAP enable the automated purification of miRNA from blood, while a novel displacement-ligation method is utilized to trigger

SUBMITTER: Benke EH 

PROVIDER: S-EPMC11700762 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature