Resolving Resident Colonic Muscularis Macrophage Diversity and Plasticity During Colitis.

ABSTRACT:

Background

Immune cell populations in the intestinal muscularis propria during colitis are poorly resolved. Maintaining homeostasis in this niche is critical, highlighted by the poorer prognosis of inflammatory bowel disease associated with muscularis propria inflammation.

Methods

This study utilizes single-cell RNA sequencing to survey the immune cell populations within the muscularis propria of normal colon and dextran sodium sulfate-induced colitis. Findings are validated by immunohistochemistry, flow cytometry and cell-lineage tracing in vivo, and in vitro assays with muscularis macrophages (MMφ).

Results

In naïve conditions, transcriptional duality is observed in MMφs with 2 major subpopulations: conventional resident Cx3cr1+ MMφs and Lyve1+ MMφs. The Lyve1+ popu

SUBMITTER: Ohishi K 

PROVIDER: S-EPMC11701110 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature