The HECT ubiquitin-protein ligases UPL1 and UPL2 are involved in degradation of Arabidopsis thaliana ACC synthase 7.

ABSTRACT: Ethylene is an important plant hormone whose production relies on the action of key enzymes, one of which is 1-aminocyclopropane-1-carboxylate synthase (ACS). There are three classes of ACS, which are all partially regulated by degradation through the ubiquitin-proteasome system (UPS), which regulates ethylene production. Arabidopsis has a single class III ACS, ACS7, but although it is known to be degraded by the 26S proteasome, the UPS proteins involved are poorly characterised. In this work, we used mass spectrometry to identify novel components of the ubiquitin system that may contribute to the regulation of ethylene biosynthesis via ACS7. We found two HECT-type ligases, UPL1 and UPL2, which regulate ACS7 stability. In vitro experiments showed that UPL1 and UPL2 E3 ligases directly cont

SUBMITTER: Marczak M 

PROVIDER: S-EPMC11701798 | biostudies-literature | 2025 Jan-Feb

REPOSITORIES: biostudies-literature