Molecular and spatial analysis of ganglion cells on retinal flatmounts: diversity, topography, and perivascularity.

ABSTRACT: Diverse retinal ganglion cells (RGCs) transmit distinct visual features from the eye to the brain. Recent studies have categorized RGCs into 45 types in mice based on transcriptomic profiles, showing strong alignment with morphological and electrophysiological properties. However, little is known about how these types are spatially arranged on the two-dimensional retinal surface-an organization that influences visual encoding-and how their local microenvironments impact development and neurodegenerative responses. To address this gap, we optimized a workflow combining imaging-based spatial transcriptomics (MERFISH) and immunohistochemical co-staining on thin flatmount retinal sections. We used computational methods to register en face somata distributions of all molecularly defined

SUBMITTER: Tsai NY 

PROVIDER: S-EPMC11702564 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature