Elucidating lipid nanoparticle properties and structure through biophysical analyses.

ABSTRACT: Designing lipid nanoparticle (LNP) delivery systems with specific targeting, potency and minimal side effects is crucial for their clinical use. However, traditional characterization methods, such as dynamic light scattering, cannot accurately quantify physicochemical properties of LNPs and how they are influenced by the lipid composition and mixing method. Here we structurally characterize polydisperse LNP formulations by applying emerging solution-based biophysical methods that have higher resolution and provide biophysical data beyond size and polydispersity. These techniques include sedimentation velocity analytical ultracentrifugation, field-flow fractionation followed by multi-angle light scattering, and size-exclusion chromatography in-line with synchrotron small-angle X-ray scatter

SUBMITTER: Padilla MS 

PROVIDER: S-EPMC11702722 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature