Of cells and tissues: Identifying the elements of a diabetic cardiac in vitro study model.

ABSTRACT: This study aimed to elucidate the impact of advanced glycation end products (AGEs) and glucose shock on cardiomyocyte viability, gene expression, cardiac biomarkers, and cardiac contractility. Firstly, AGEs were generated in-house, and their concentration was confirmed using absorbance measurements. AC16 cardiomyocytes were then exposed to varying doses of AGEs, resulting in dose-dependent decreases in cell viability. The maximum tolerated dose of AGEs was determined, revealing significant downregulation of the cardiac gene gap junction alpha 1 (GJA1). Furthermore, the study assessed the effects of AGEs, glucose shock, and their combination on biomarkers, cardiac myosin heavy chain (MHC), and connexin-43 (Cx-43) in AC16 cells. It was found that AGEs supplementation induced an increase in M

SUBMITTER: Hernandez I 

PROVIDER: S-EPMC11702775 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature