Triple coding in human SRD5A1 mRNA.

ABSTRACT:

Background

Nucleotide sequence can be translated in three reading frames from 5' to 3' producing distinct protein products. Many examples of RNA translation in two reading frames (dual coding) have been identified so far.

Results

We report simultaneous translation of mRNA transcripts derived from SRD5A1 locus in all three reading frames that result in the synthesis of long proteins. This occurs due to initiation at three nearby AUG codons occurring in all three-reading frame. Only one of the three proteoforms contains the conserved catalytical domain of SDRD5A1 produced either from the second or the third AUG codon depending on the transcript. Paradoxically, ribosome profiling data and expression reporters indicate that the most efficient translation produces catalyti

SUBMITTER: Yordanova MM 

PROVIDER: S-EPMC11702784 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature