Optimizing encephalomyocarditis virus VP1 protein assembly on pseudorabies virus envelope via US9 protein anchoring.

ABSTRACT: Live herpesvirus-vectored vaccines are critical in veterinary medicine, but they can sometimes offer insufficient protection due to suboptimal antigen expression or localization. Encephalomyocarditis virus (EMCV) is a significant zoonotic threat, with VP1 protein as a key immunogen on its capsid. To enhance immunogenicity, we explored the use of recombinant pseudorabies virus (rPRV) as a vaccine vector against EMCV. In silico analysis indicated that fusing VP1 with US9 enhances the formation of a type II transmembrane heterodimer. We constructed six rPRV groups expressing different VP1 variants and found that VP1 fused with US9's C-terminal (US9-VP1) enhances VP1's membrane localization and its incorporation into the PRV envelope, unlike wild-type VP1. Immunogold electron microscopy illust

SUBMITTER: Ren BR 

PROVIDER: S-EPMC11702956 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature