Probing mechanical selection in diverse eukaryotic genomes through accurate prediction of 3D DNA mechanics.

ABSTRACT: Connections between the mechanical properties of DNA and biological functions have been speculative due to the lack of methods to measure or predict DNA mechanics at scale. Recently, a proxy for DNA mechanics, cyclizability, was measured by loop-seq and enabled genome-scale investigation of DNA mechanics. Here, we use this dataset to build a computational model predicting bias-corrected intrinsic cyclizability, with near-perfect accuracy, solely based on DNA sequence. Further, the model predicts intrinsic bending direction in 3D space. Using this tool, we aimed to probe mechanical selection - that is, the evolutionary selection of DNA sequence based on its mechanical properties - in diverse circumstances. First, we found that the intrinsic bend direction of DNA sequences correlated with th

SUBMITTER: Park J 

PROVIDER: S-EPMC11703244 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature