A signaling inspired synthetic toolkit for efficient production of tyrosine phosphorylated proteins.

ABSTRACT: Tyrosine phosphorylation is an important post-translational modification that regulates many biochemical signaling networks in multicellular organisms. To date, 46,000 tyrosines have been observed in human proteins, but relatively little is known about the function and regulation of most of these sites. A major challenge has been producing recombinant phosphoproteins in order to test the effects of phosphorylation. Mutagenesis to acidic amino acids often fails to replicate the size and charge of a phosphorylated tyrosine residue and synthetic amino acid incorporation has high cost with relatively low yield. Here, we demonstrate an approach, inspired by how native tyrosine kinases find targets in cells - through a secondary targeting interaction, augmenting innate catalytic specificity of a

SUBMITTER: Ryan MM 

PROVIDER: S-EPMC11703254 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature