Premature termination of RNA polymerase II mediated transcription of a seed protein gene in Schizosaccharomyces pombe.

ABSTRACT: The poly(A) signal and downstream elements with transcriptional pausing activity play an important role in termination of RNA polymerase II transcription. We show that an intronic sequence derived from the plant seed protein gene (AmA1) specifically acts as a transcriptional terminator in the fission yeast, Schizosaccharomyces pombe. The 3'-end points of mRNA encoded by the AmA1 gene were mapped at different positions in S.pombe and in native cells of Amaranthus hypochondriacus. Deletion analyses of the AmA1 intronic sequence revealed that multiple elements essential for proper transcriptional termination in S.pombe include two site-determining elements (SDEs) and three downstream sequence elements. RT-PCR analyses detected transcripts up to the second SDE. This is the first report showing

SUBMITTER: Chakraborty S 

PROVIDER: S-EPMC117036 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature