Conductive Microfibers Improve Stem Cell-Derived Cardiac Spheroid Maturation.

ABSTRACT: Conventional two-dimensional (2D) cardiomyocyte differentiation protocols create cells with limited maturity, which impairs their predictive capacity and has driven interest in three-dimensional (3D) engineered cardiac tissue models of varying maturity and scalability. Cardiac spheroids are attractive high-throughput models that have demonstrated improved functional and transcriptional maturity over conventional 2D differentiations. However, these 3D models still tend to have limited contractile and electrical maturity compared to highly engineered cardiac tissues; hence, we incorporated a library of conductive polymer microfibers in cardiac spheroids to determine if fiber properties could accelerate maturation. Conductive microfibers improved contractility parameters of cardiac spheroids

SUBMITTER: Gonzalez G 

PROVIDER: S-EPMC11703634 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature