Novel rhesus macaque immunoglobulin germline genes identified by three sequencing approaches.

ABSTRACT:

Introduction

Rhesus macaques have long been a focus of research for understanding immune responses to human pathogens due to their close phylogenetic relationship with humans. As rhesus macaque antibody germlines show high degrees of polymorphism, the spectrum of database-covered genes expressed in individual macaques remains to be determined.

Methods

Here, four rhesus macaques infected with SHIV_SF162P3N became a study of interest because they developed broadly neutralizing antibodies against HIV-1. To identify the immunoglobulin heavy chain V-gene (IGHV) germlines in these macaques, we applied three sequencing approaches - genomic DNA (gDNA) TOPO sequencing, gDNA MiSeq, and messenger RNA (mRNA) MiSeq inference with IgDiscover, and illustrated the detection power

SUBMITTER: Guo Y 

PROVIDER: S-EPMC11703713 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature