The bioinformatics analysis and experimental validation of the carcinogenic role of EXO1 in lung adenocarcinoma.

ABSTRACT:

Background

Exonuclease 1 (EXO1), a protein involved in mismatch repair and recombination processes, has been identified as a prognostic biomarker in lung adenocarcinoma (LUAD). Nevertheless, its role in LUAD progression remains elusive. This study seeks to elucidate the functional significance of EXO1 in LUAD and evaluate its potential as a therapeutic target.

Materials and methods

Patient RNA-seq and clinical data were acquired from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Subsequently, a protein-protein interaction (PPI) network was constructed using differentially expressed genes (DEGs) to identify pivotal genes. Validation of the expression of signature genes was carried out through quantitative real-time PCR (qRT-PCR). Additionally, th

SUBMITTER: Sun B 

PROVIDER: S-EPMC11703735 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature