Role of p57KIP2 in Stem and Progenitor Leydig Cells of Mouse Testes.

ABSTRACT:

Purpose

Precise control of proliferation and differentiation of Leydig cells is important for gonadal androgenesis and spermatogenesis. Though cyclin-dependent kinase inhibitors are crucial for cell proliferation and differentiation, their role in the development of early adult Leydig cells (ALCs) remained unanswered. To understand mechanism for ALC development, functional expression of p57KIP2 (cdkn1c) was investigated in the stem Leydig cells (SLCs) and progenitor Leydig cells (PLCs) in mice.

Materials and methods

The roles of p57KIP2 in the proliferation, differentiation, apoptosis, and steroidogenesis in SLCs and PLCs were investigated by antibodies and bromodeoxyuridine (BrdU) labeling in the early neonatal testes and p57^kip2 siRNA in the isolated SLCs

SUBMITTER: Park SH 

PROVIDER: S-EPMC11704159 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature