Bisphenol Analogs Downregulate the Self-Renewal Potential of Spermatogonial Stem Cells.

ABSTRACT:

Purpose

In this study, we investigated the effect of bisphenol-A (BPA) and its major analogs, bisphenol-F (BPF), and bisphenol-S (BPS), on spermatogonial stem cells (SSCs) populations using in vitro SSC culture and in vivo transplantation models.

Materials and methods

SSCs enriched from 6- to 8-day-old C57BL/6-eGFP⁺ male mice testes were treated with varying concentrations of bisphenols for 7 days to examine bisphenol-derived cytotoxicity and changes in SSC characteristics. We utilized flow cytometry, immunocytochemistry, real-time quantitative reverse transcription-PCR, and western blot analysis. The functional alteration of SSCs was further investigated by examining donor SSC-derived spermatogenesis evaluation through in vivo transplantation and subseq

SUBMITTER: Kim SH 

PROVIDER: S-EPMC11704178 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature