Alternative splicing in the DBD linker region of p63 modulates binding to DNA and iASPP in vitro.

ABSTRACT: The transcription factor p63 is expressed in many different isoforms as a result of differential promoter use and splicing. Some of these isoforms have very specific physiological functions in the development and maintenance of epithelial tissues and surveillance of genetic integrity in oocytes. The ASPP family of proteins is involved in modulating the transcriptional activity of the p53 protein family members, including p63. In particular, iASPP plays an important role in the development and differentiation of epithelial tissues. Here we characterize the interaction of iASPP with p63 and show that it binds to the linker region between the DNA binding domain and the oligomerization domain. We further demonstrate that this binding site is removed in a splice variant of p63 where a stretch o

SUBMITTER: Lotz R 

PROVIDER: S-EPMC11704248 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature