Single-Cell RNA sequencing reveals mitochondrial dysfunction in microtia chondrocytes.

ABSTRACT: Microtia is a congenital malformation characterized by underdevelopment of the external ear. While chondrocyte dysfunction has been implicated in microtia, the specific cellular abnormalities remain poorly understood. This study aimed to investigate mitochondrial dysfunction in microtia chondrocytes using single-cell RNA sequencing. Cartilage samples were obtained from patients with unilateral, non-syndromic microtia and healthy controls. Single-cell RNA sequencing was performed using the 10 × Genomics platform. Bioinformatic analyses including cell type identification, trajectory analysis, and gene co-expression network analysis were conducted. Mitochondrial function was assessed through ROS levels, membrane potential, and transmission electron microscopy. Chondrocytes from microtia sampl

SUBMITTER: Li X 

PROVIDER: S-EPMC11704343 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature