Perturbations in the gut microbiome of C57BL/6 mice by the sobriety aid Antabuse® (disulfiram).

ABSTRACT:

Aims

Disulfiram (Antabuse®) is an oral alcohol sobriety medication that exhibits antimicrobial activity against Gram-positive facultative anaerobes. The aims of this study were to measure the antimicrobial activity against anaerobic bacteria of the gut human microbiome and establish the extent that disulfiram alters the microbial composition of the ileum, cecum, and feces using C57BL/6 mice.

Methods and results

Antimicrobial susceptibility testing by the microdilution method revealed that disulfiram inhibits the in vitro growth of gut anaerobic species of Bacteroides, Clostridium, Peptostreptococcus, and Porphyromonas. Differential sequencing of 16S rRNA isolated from the ileum, cecum, and feces contents of treated vs. untreated mice showed that disulfiram enriches the Gram-

SUBMITTER: Evans SE 

PROVIDER: S-EPMC11704607 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature